Fig. 8

The charge of E313 and R432 is essential for lipid scrambling. a–f Time course of dithionite-induced fluorescence decay for the indicated nhTMEM16 mutants of E313 and R432 in the presence (red) and absence (black) of Ca²⁺. Dashed yellow lines indicate fits to Eq. (6). Protein-free traces are shown in green. g, h Quantification of scrambling rate constants α and β for WT and mutant nhTMEM16 in the presence (g) and absence of Ca²⁺ (h). The effects were categorized as in Fig. 2g, h. Individual data points are shown as empty circles. i Fraction of the liposomes containing at least one active channel in the presence (red) and absence of Ca²⁺ (black). All data is reported as the mean ± S.D. The number of replicates are indicated in Supplementary Table 1