Fig. 3
From: JunB defines functional and structural integrity of the epidermo-pilosebaceous unit in the skin
Impaired wound healing and stress response in JunB cKO mice. a JunB cKO mice display delayed wound healing compared to wild type mice following 6 mm full-thickness circular biopsy punch excision. **P < 0.001, one-way ANOVA (n = 7) b Representative H&E photomicrographs of a 10 days wound depicting a hyperplastic and thickened epidermis and enlarged sebaceous glands in JunB cKO mice compared to wild type mice. Scale bars, 50 µm. c Enhanced epidermal and sebaceous gland hyperplasia in JunB cKOs at day 5 following hair plucking. Inset image depicts opposing role of JunB (red) on proliferating cells marked in green with Ki67. Quantification of the size of sebaceous glands following hair plucking, ***P < 0.001, t-test (n = 3). d The topical application of TPA, a strong enhancer of proliferation, for 7 days markedly triggered epidermal and sebaceous gland hyperplasia. Quantification of the size of sebaceous glands after TPA application, ***P < 0.001, t-test (n = 3). E, epidermis; D, dermis; HF, hair follicle; SG, sebaceous gland; SC, subcutaneous layer; PC, panniculus carnosus. Scale bars, 50 µm
