Fig. 4
From: JunB defines functional and structural integrity of the epidermo-pilosebaceous unit in the skin
De novo formation of sebaceous glands in JunB cKO wounds. a Representative H&E photomicrographs of 30 days old wound restoration tissue depicting ectopic sebaceous glands in JunB cKO mice. Inset showing magnified view. E, epidermis; SG, sebaceous gland. Scale bars, 50 µm. Quantification of de novo sebaceous gland numbers in 30 days old wounds. ***P < 0.001, t-test (n = 3). Graphical sketch summarizes JunB-dependent suppression of epidermal progenitor differentiation towards sebocyte lineage (left bottom panel as opposed to lineage plasticity of epidermal progenitors towards sebocyte differentiation (right bottom panel). b, c Representative microphotographs with immunostaining of SCD1 (red), indicative of sebocyte differentiation, demonstrating different stages of de novo formation of sebaceous glands in 30 days old wounds in JunB cKO mice. Nuclei stained with DAPI in blue. d Representative photomicrographs displaying the formation of elongated sebaceous duct in 90-day-old wound epithelium in JunB cKO mice. Wound tissues were stained either with H & E or the sebaceous glands specific marker FABP5 (red) and the basal layer specific keratin K14 (green). Nuclei stained with DAPI in blue. Scale bars, 50 µm. E, epidermis; SG, sebaceous gland
