Fig. 1
Clues of elevated expressions of PBOV1 and EGFR in HCC clinical specimens and cell lines. a Immunohistochemical stain (IHC) analysis of prostate and breast cancer overexpressed 1 (PBOV1) protein expression in each of the hepatocellular carcinoma (HCC) tissues (T) and adjacent nontumor tissue liver tissues (ANT) paired from the same patient. Scale bar, 100 μm. b Western blotting analysis of PBOV1 protein expression in HCC tissues and paired ANT liver tissues from the same patient. c Overall survival of HCC patients with low PBOV1 expression versus patients with high PBOV1 expression (n = 286, P < 0.05, log-rank). d Disease-free survival of HCC patients with low PBOV1 expression versus patients with high PBOV1 expression (n = 286, P < 0.05, log-rank). e Expressions of PBOV1 and epidermal growth factor receptor (EGFR) determined by western blotting in the human normal hepatocyte cell lines (L02, HHL-5) and multiple HCC cell lines (Hep3B, BEL7402, SNU423, SMMC-7721, PLC/PRF/5, Huh7, HLE, HLF, HepG2.2.1, SK-Hep1, and HepG2). f Confocal laser scanning microscopy (CLSM) imaging of Hep3B, BEL7402, and SMMC-7721 cells via EGFR immunofluorescence staining with EGFR-IgG antibody or Isotype control antibody. Scale bar, 10 μm. (Blue, DAPI-indicating nuclei; Green, FITC indicating EGFR primary antibody aggregation). (See also Supplementary Fig. 1)
