Fig. 5

Blimp-1 expression identifies two populations of SPF cells. a Maximum intensity projection (360 × 360 × 20 μm) 5 days after HEL-OVA secondary immunisation. SW_HEL B cells (red), Blimp-1⁺ SW_HEL B cells (yellow, from co-expression of red and green), Tfh cells (cyan), CD169⁺ SCS macrophages (pink), SHG (blue). Scale bar = 50 μm. b Cell tracking analysis of a. Blimp-1⁺ SW_HEL B cell tracks (yellow), Blimp -1^neg SW_HEL B cell tracks (red) in the SPF. See also Supplementary Movie 6. Scale bar = 50 μm. c CDF plot of speed for Blimp-1⁺ (blue) and Blimp -1^neg SW_HEL B cells (red). d CDF plot of displacement Blimp-1⁺ (blue) and Blimp -1^neg SW_HEL B cells (red). e CDF plot of meandering index for Blimp-1⁺ (blue) and Blimp -1^neg SW_HEL B cells (red). KS statistics refer to Kolmogorov–Smirnov statistics. f Mean squared displacement (MSD) plot of Blimp-1⁺ (blue) and Blimp -1^neg SW_HEL B cells (red). g Representative tracks for Blimp-1⁺ and Blimp -1^neg cells in the SPF. Data are representative of 5 independent experiments