Fig. 6
From: Memory B cells are reactivated in subcapsular proliferative foci of lymph nodes
Deconvolution of the secondary antibody response by single-cell RNA sequencing. a Heatmap showing 8190 differentially expressed genes from 122 single SWHEL donor-derived B cells. b Deconvolution of 122 single cells into three populations by expression of metagenes 1, 2 and 3 by NMF. c Vector diagram showing individual gene contributions to the metagenes 1, 2 and 3. d GSEA identifies population 1 as GC. e GSEA identifies population 2 as plasma cell. f GSEA identifies population 3 as MBCs. g Flow cytometric analysis of donor-derived HEL-binding B cells 4 days after HEL-OVA re-immunisation showing CD38, intracellular (IC) HEL-binding immunoglobulin and Bcl-6 expression on the 3 populations. h Pseudotime analysis of single-cell RNA sequencing data. i Proportion of 3 populations by flow cytometric analysis of donor-derived HEL-binding B cells 5 and 7 days after HEL-OVA re-immunisation. Data are representative of two independent experiments with 5 mice per time point
