Fig. 5
Anterior/posterior localization of IT-type and PT-type corticostriatal projections. a Anterior/posterior location of suprathreshold voxels in ipsilateral striatum was quantified for IT-type and PT-type injection cases. Mean ± SEM plotted. IT-type projections are highlighted in color (for example, vS1 is teal). Corresponding PT-type projections from the same cluster are plotted in black on the same axes. Number of suprathreshold voxels is similar for vS1, orfS1, and fS1 injections. Suprathreshold voxels for IT-type projections from frontal areas exceed those of PT-type projections. b Peak normalized distribution (Mean ± SEM) of IT-type and PT-type projections are shown. These peak at similar points on the anterior/posterior axis. c To assess differences in targeting of IT-type and PT-type projections within the same injection site cluster, the center of mass of corticostriatal fluorescence of the voxels for the mean normalized injection pattern was calculated for each injection site cluster. Overall center of mass is shown as a large circle (red (vS1 IT-type) and green (vS1 PT-type) circles, example at bottom). Corticostriatal fluorescence center of mass of each plane is plotted as a circle, and projections along the x/y/z-axes are shown. Size of the circle is proportional to the summed normalized voxel intensity for a given plane. Anterior/posterior projections for each injection cluster are shown above. Color code corresponds to the injection site cluster (teal for vS1), with IT-type projections shown in color and corresponding PT-type projections shown in black. Dotted line is shown for anterior/posterior alignment across injection clusters. Corticostriatal fluorescence center of mass of vS1, orfS1, and fS1 (teal, gray, and gold, respectively) are posterior within the striatum, while frontal areas ALM and M2 (orange and red) are anterior. Overall center of mass of corticostriatal fluorescence overlaps for IT- and PT-type cases, resulting in overlap of these markers. Scale, 1 mm (bottom right)
