Fig. 7
Single neuron reconstructions of IT-type and PT-type neurons in motor areas. a Reconstruction of the long-range axonal projections of adjacent PT-type (gold) and IT-type (blue) L5 pyramidal neurons34. Projections throughout the whole CNS anterior to medulla are shown. The outline of the reference brain is shown. Scale, 1 mm. b Somata are in adjacent in primary motor cortex (M1). In coronal sections (b, c, e, f, h, i), the CCF reference atlas regions are shown in grayscale, with each area or cortical layer for a given cortical area shown in a distinct shade of gray. Scale, 0.5 mm. c Corticostriatal projections show similar general topography with differences in arbor size and density. Scale, 0.5 mm. d Five adjacent IT-type (red, teal, and gold) and PT-type (green and white) M1 neurons. PT-type neurons shown arborize in thalamus, superior colliculus, pons, and medulla in addition to ipsilateral striatum. The 3D outline of the reference brain is shown, with ipsi- and contralateral striatum highlighted in light gray. e, f Corticostriatal projections show topography of IT-type and PT-type projections, as well as differences in density, terminal field size, and asymmetry of projections to contralateral striatum. White box in e magnified in f. Scale, 1 mm (e), 0.5 mm (f). (g–i) Five adjacent IT-type (purple, teal, and blue) and PT-type (green and off-white) secondary motor cortex (M2) neurons. Scale, 1 mm (h), 0.5 mm (i)
