Fig. 1 | Nature Communications

Fig. 1

From: The VAR2CSA malaria protein efficiently retrieves circulating tumor cells in an EpCAM-independent manner

Fig. 1

rVAR2 binds specifically to a diverse repertoire of cancer cells. a Detection of cancer cells using the CytoTrack platform. Representative confocal microscopy images of indicated cell lines. Cancer cells were mixed with PBMCs in a 1:5000 ratio prior to analysis and stained with His-tagged rVAR2 in combination with anti-penta His Alexa Fluor 488 (green), an anti-CD45 Cy5 antibody (red), and DAPI (blue). Scale bars, 10 µm. b Flow cytometry measured fluorescence intensity of three breast cancer (left panel), three prostate cancer (middle panel), and three colorectal cancer (right panel) cell lines stained by His-tagged rVAR2 in combination with anti-penta His Alexa Fluor 488 (y-axis) and a PE-conjugated anti-EpCAM antibody (x-axis)

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