Fig. 3
rVAR2-based CTC isolation using magnetic beads. a Representative confocal microscopy image of a PC3 cell captured by rVAR2-coated magnetic beads in combination with the IsoFlux™ device after spiking into 5 mL of healthy donor blood. Isolated cells were incubated with anti-cytokeratin FITC antibody (green), anti-CD45 PE antibody (red), and DAPI (blue). Scale bars, 10 µm. b A low-passage pancreatic ductal adenocarcinoma (PDAC) cell from a patient-derived xenograft captured by rVAR2-coated magnetic beads in combination with the IsoFlux™ device after spiking into 5 mL of healthy donor blood. Isolated cells were incubated with anti-cytokeratin FITC antibody (green), anti-CD45 PE antibody (red), and DAPI (blue). Scale bars, 10 µm. c CTC isolation efficiency by spike-in experiments; 100 PC3 cells or PDAC cells were spiked into 5 mL of blood and isolated by rVAR2-coated beads in combination with the IsoFlux™. The recovery was estimated by immunofluorescence staining of CK+, CD45−, DAPI+ cells (mean ± s. d., n = 3). d Recovery of PDAC cells spiked into 5 mL of blood. The number of spiked PDAC cells (10, 20, 50, 100, or 200 cells) is plotted versus the number of PDAC cells isolated by rVAR2-coated beads (n = 5). Equation shows a linear regression model
