Table 3 KRAS mutational analysis of total DNA extract from CTC isolates from pancreatic cancer patient blood samplesa

From: The VAR2CSA malaria protein efficiently retrieves circulating tumor cells in an EpCAM-independent manner

IDa

CTCsb

PBMCsc

KRAS mutation

Mutated KRAS genes (copies per µL)d

Patient 1

68

1170

G12D

0.94

Patient 2

87

1885

G12D

1.03

Patient 3

90

1075

G12V

1.05

Patient 4

107

1072

G12D

1.13

  1. aBlood samples derive from the same pancreatic cancer patients as in Fig. 4a
  2. bCTCs were defined as CK+, DAPI+, CD45−
  3. cPBMCs were defined as CK−, DAPI+, CD45+
  4. dFor the analysis of each patient sample, 100 PDAC cells were spiked into 5000 PBMCs prior to the DNA extraction procedure and run in parallel as a positive control, resulting in values between 0.93 and 0.94 copies of mutated KRAS genes per microliter
Back to article page