Fig. 3
From: Utilization of rare codon-rich markers for screening amino acid overproducers
Cell growth restored by feeding the corresponding l-amino acids. a Effects of feeding l-leucine or a mixture of three amino acids (3AA: l-leucine, l-valine and l-isoleucine) on the cell growth for E. coli strains harboring kanR genes with 6−29 leucine rare codons (kanR-RC6, kanR-RC16, kanR-RC26, kanR-RC29). b Changes in cell ODs after feeding l-arginine to E. coli and C. glutamicum strains harboring kanR genes in which eight arginine codons were replaced by its rare alternatives (encoded by pAKR-RC8 or pKan-CG-RC8), and the growth restoration after feeding l-serine to E. coli strains carrying specR which was rich in serine rare codon (encoded by pSSer-RC17). Values and error bars represent the mean and the s.d. (n = 3). **P < 0.01, ***P < 0.001 as determined by two-tailed t test
