Fig. 4
From: Utilization of rare codon-rich markers for screening amino acid overproducers
Selection stringency mediated by copy number and promoter strength. The kanR, kanR-RC29, and kanR-RC8 represent the wild-type kanR, the kanR containing leucine rare codon CTA, and the kanR containing arginine rare codon AGG, respectively. a The effects of different ORIs on the cell OD600 for strains harboring the wild-type and the rare codon-rich markers. b The effects of constitutive and inducible promoters on the cell OD600 for strains harboring the rare codon-rich markers, the dashed line represents the cell OD600 for strain carrying the wild-type kanR driven by its original promoter from pET-28a. Values and error bars represent the mean and the s.d. (n = 3)
