Fig. 6 | Nature Communications

Fig. 6

From: Utilization of rare codon-rich markers for screening amino acid overproducers

Fig. 6

Transcription profiles of BCAA biosynthetic pathways in LP-4 and LP-7. a Transcriptome analysis of LP-4, LP-7, and the wild-type strains. Positive values (red) represent upregulated genes and negative values (blue) represent downregulated genes, which were calculated by the RPKM of LP-4 and LP-7 divided by that of the wild-type strain (in log2). The top, middle, and bottom panels contain genes in glycolysis, l-leucine biosynthesis and BCAA transportations, respectively. b The BCAA biosynthetic pathways in E. coli, red and blue arrows indicate the up- and downregulated genes, respectively. c qRT-PCR verification of the genes related to l-leucine biosynthesis and transportation. Values and error bars represent the mean and the s.d. (n = 3)

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