Fig. 4
Integrated bioinformatics of MSC secretome proteomics with transcriptomics of blood ASC and BM LLPC. a Flow diagram of the integrated bioinformatic analysis. b Proteomics of distinct MSC secretome fractions. Three fractions strongly support blood ASC survival (blue symbols): iMSC secretome (blue filled square; left and right panels), noniMSC secretome (blue open triangles; left panel), and supernatant fractionated by ultracentrifugation of iMSC secretome (blue open circles; left panel); and three fractions with decreased ASC survival (black symbols): conventional media (R10) (black open circles; left panel), supernatant fractionated by overnight ultracentrifugation of noniMSC secretome (black open triangles; left panel), and secretome from blood (not BM) adherent cells (black open triangles; right panel). c A heat map of the 2558 DEG between 17 blood ASC (PB ASC) obtained from seven healthy subjects and BM LLPC (LLPC) obtained from four adult subjects. d HIPPIE analysis of 91 MSC protein revealed 4429 potential protein partners (PPI). Overlap of the 4429 PPI with the 2558 DEG uncovered 556 overlapping gene/protein targets and led to 20 statistically significant GSEA hallmark pathways. e Of the aforementioned 91 MSC secretome proteins, FN-1 and YWHAZ had the highest number (118 and 119, respectively) of potential interacting partners. Of these potential partners, 31 were shared between both FN-1 and YWHAZ. f From the 20 GSEA pathways, FN-1 and YWHAZ were found to be involved in these 10 potential GSEA hallmark pathways
