Fig. 5
Validation of ASC survival. Treatment of MSC secretome with antibodies targeting a FN-1 or b YWHAZ diminishes blood ASC survival. Blood ASC were cultured in MSC secretome alone (white bar) or MSC secretome treated with antibodies targeting FN-1 (a; black bar) (p < 10-3; linear regression modeling using media (secretome) as a covariate) or YWHAZ (b; black bar) (p < 10-5; linear regression modeling using media (secretome) as a covariate), or appropriate isotype controls (gray bar). Graph represents average of at least three experiments. Sensitivity and resistance to Rapamycin in c blood ASC and d BM LLPC. Blood ASC from three healthy subjects or BM LLPC from three adults were sorted and cultured in untreated MSC secretome (black bar) or MSC secretome treated with rapamycin (gray bar). The frequency of vehicle controls (DMSO) was comparable to that of untreated MSC secretome. p = 0.001; ANOVA) (c); NS, non-significance (d)
