Fig. 2
From: FOXD1-dependent MICU1 expression regulates mitochondrial activity and cell differentiation
MICU1 is transcriptionally regulated by Foxd1 under hypoxia. a Quantification of MICU1 mRNA abundance in hiPSCs exposed to normoxia (20% O2) and hypoxia (5% O2). b Representative western blot for lysates from control fibroblasts and hiPSCs grown under hypoxic/normoxic conditions and probed with antibody specific for MICU1, MCU, ATP5A, and Tom20. c, d Quantification of relative MICU1 (c) and MCU (d) protein abundance in hiPSCs grown under hypoxic/normoxic conditions. e Quantification of Foxd1 mRNA abundance in hiPSCs under normoxia/hypoxia. f Representative western blot for lysates from control fibroblasts and hiPSCs grown under normoxic/ hypoxic conditions and probed with antibody specific for Foxd1 and β-actin. g Quantification of relative protein abundance of Foxd1 and MICU1 quantified from f and b. h Quantification of Foxd1 mRNA abundance in various tissues (brain, heart, liver, and lung) harvested from different stages of embryonic development. i Representative western blot for lysates from brain, heart, liver, and lung harvested from embryos/neonates and probed with antibodies specific for MICU1, Foxd1, and β-actin. j Quantification of relative protein abundance of Foxd1 and MICU1 quantified from i. k ChIP-assay was performed in hiPSCs exposed to normoxia and hypoxia. Antibody specific for Foxd1 was used to immunoprecipitate the chromatin and the fold enrichment of micu1 promoter relative to the matched input control was quantified by qRT-PCR. Bar represents Mean ± SEM, **P < 0.01, *P < 0.05, ***P < 0.001; n = 3–5 (One-Way ANOVA)
