Fig. 4
From: mTOR-dependent phosphorylation controls TFEB nuclear export
Hierarchical phosphorylation controls TFEB nuclear export. a Representative time-lapse images of HeLa cells transfected with either wild-type TFEB-GFP or with TFEB mutants S142A, S138A, and S211A. Twenty-four hours after transfection, cells were subjected to FRAP experiments. Scale bar: 10 μm. b Cells described in a were analyzed and plotted for the decay in TFEB nuclear fluorescence. Results are mean ± SEM. n > 7 cells per condition. c HeLa cells were transfected with either wild-type or serine-to-alanine mutant TFEB for 24 h and evaluated for TFEB phosphorylation by immunoblotting using the indicated antibodies
