Fig. 3

Loss of Krt76 results in local and circulating increase in cytokines and expansion of Tregs. a, b Summary of flow cytometric analysis of % effector T cells (CD44^high CD62L^low) (a) and Foxp3⁺ Tregs (b) in total TCRβ⁺ CD3⁺ CD4⁺ T cells in thymus (Thy), spleen (Spl) and lymph nodes (LN; sLN, submandibular lymph nodes) from control and Krt76^−/− mice (n = 4 mice/genotype, mean ± s.e.m., unpaired t-test). c, d Levels of cytokines in blood serum (c) and cyst fluid (d) of control and Krt76^−/− mice assessed by CBA analysis (n = 4 mice per genotype; experiment repeated twice, mean ± s.e.m., unpaired t-test). The same blood serum measurements for Krt76^−/− mice are shown in c and d. e Quantitative RT-PCR of cytokine mRNAs, relative to Gapdh, in tongue and squamous stomach epithelia (n = 4 mice/genotype, mean ± s.e.m. of biological and technical triplicates; unpaired t-test). f, g Representative images of CD45 (green) and Keratin14 (red) stained sections of skin, tongue and squamous stomach epithelia (f), and quantification of infiltrating CD45⁺ leucocytes/mm² of stromal region (g). Stromal region corresponds to area between the epithelium and the white dotted line; n = 3 mice per condition, 2 sections per mouse and >4 microscopic views per mice, means ± s.e.m., ***p ≤ 0.001, ****p ≤ 0.0001, unpaired t-test). *p ≤ 0.05; **p ≤ 0.01; ***p ≤ 0.001; ***p ≤ 0.001; ns non-significant. Scale bars: 100 µm