Fig. 4
From: Reprogramming of the antimycin NRPS-PKS assembly lines inspired by gene evolution
Ring expansion of JBIR-06. a The reconstructed module structure for ring expansion. b HPLC analyses of the metabolites from transformants harboring (i) pKU518J06 and (ii) pKU518J06ΔsmlCTE/pZH2-NatD. The chromatogram represents the UV absorbance at 320 nm. *** indicates a mixture of analogs with m/z values that are 14 less than 5. c Engineering scheme and structure of 5 and 6
