Fig. 5
Blimp-1 mediates butyrate induction of IL-10 production in Th1 cells. a WT CBir1 (WT) and Gpr43−/– CBir1 Tg (Gpr43KO) Th1 cells (n = 3/group) were cultured with APC and CBir1 peptide in the presence or absence of C4 for 5 days. The expression of Blimp-1 was examined by qRT-PCR. b WT CBir1 Th1 cells were transfected with Prdm1 siRNA or control siRNA. After 24 h of transfection, the cells were treated with or without C4, and IL-10 expression was determined by qPCR. c–f WT and Prdm1−/− Th1 cells were cultured with APC and anti-CD3 mAb in the presence or absence of C4. Expression of IL-10, IFN-γ, and Foxp3 was determined by flow cytometry. qRT-PCR results are shown as mean ± SD of triplicates, and FACS profiles are a representative of triplicates from one of three experiments performed. *p < 0.05, **p < 0.01, ***p < 0.001 one-way ANOVA test
