Fig. 8 | Nature Communications

Fig. 8

From: Microbiota-derived short-chain fatty acids promote Th1 cell IL-10 production to maintain intestinal homeostasis

Fig. 8

Butyrate promotes IL-10 production by T-cells from patients with IBD. CD4+ T-cells from peripheral blood mononuclear cells from IBD patients of CD (n = 6), UC (n = 6), and healthy individuals (HC, n = 6) were cultured with anti-CD3 mAb and anti-CD28 mAb in the presence of C4 for 5 days. a CD4+ T-cell cytokine production and Foxp3 expression were determined by flow cytometry. b Bar charts of the percentage of cytokine-expressing CD4+ T-cells. c IL-10 in supernatants of cell culture and d CD4+ T-cell expression of Prdm1 were examined by ELISA and qRT-PCR, respectively. The results are shown as mean ± SD of triplicates and are representative from one of three experiments performed. *p < 0.05, **p < 0.01 Student’s t-test

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