Fig. 5

Endothelial PDGF-B promotes tuft formation and OIR revascularization. a Strategy to assess neoangiogenesis after OIR. The triangles indicate tamoxifen injection. b Retinal flat mounts after OIR. Insets show avascular area measured for quantification. Lower panels show higher-magnification images of pathological vascular tufts. c Avascular area (left) and NVT (right) quantification. Number of retinas used for quantification is indicated. Error bars represent s.e.m. ^⋆⋆P < 0.01, Mann–Whitney U test. d (Left) Quantification of α-SMA (top) and PDGFRβ (bottom) coverage of IB4+ vascular tufts. (Right) IB4 and α-SMA (top) or IB4 and PDGFRβ (bottom) double staining of the NVTs. Number of retinas used for quantification is indicated. Error bars represent s.e.m. ^⋆⋆P < 0.01, Mann–Whitney U test. e (Left) Quantification of DESMIN coverage of IB4+ vascular sprouts. (Right) IB4 and DESMIN double staining of the angiogenic sprouts in P5 normoxic and P17 OIR retinas. Number of retinas used for quantification is indicated. Error bars represent s.e.m. ^⋆⋆P < 0.01, Mann–Whitney U test. f, g Images (f) and quantification (g) of TER119+ RBC leakage in Pdgf-biEC compared to control littermate mice. Number of mice used for quantification is indicated. Error bars represent s.e.m. ^⋆⋆⋆P < 0.001, Mann–Whitney U test. Scale bars, 1 mm (b top), 100 μm (b bottom), and 30 μm (d–f)