Fig. 7
From: Structural basis for importin alpha 3 specificity of W proteins in Hendra and Nipah viruses
Confirming the structural hypothesis through importin α chimeras. a Schematic representation of importin α1, α3, and chimeric importin α1ARM1-5:α3ARM6-10 and importin α3ARM1-5:α1ARM6-10 constructs. b Co-immunoprecipitation assay performed with Flag antibody on lysates of HEK293T cells expressing Flag-tagged importin α1, α3, importin α1ARM1-5:α3ARM6-10 and importin α3ARM1-5:α1ARM6-10, and HA-tagged HeV W and NiV W, as indicated. Western blots were performed for HA and Flag. WCL, whole cell lysate; IP, immunoprecipitation. pCAGGS denotes empty vector control. c Co-immunoprecipitation assay performed with HA antibody on lysates of HEK293T cells expressing Flag-tagged importin α1, α3, importin α1ARM1-5:α3ARM6-10 and importin α3ARM1-5:α1ARM6-10, and HA-tagged HeV W and NiV W, as indicated. Western blots were performed for HA and Flag. WCL, whole-cell lysate; IP, immunoprecipitation. pCAGGS denotes empty vector control
