Fig. 10 | Nature Communications

Fig. 10

From: CFP1 coordinates histone H3 lysine-4 trimethylation and meiotic cell cycle progression in mouse oocytes

Fig. 10

Inhibition of CFP1 phosphorylation and degradation blocks preimplantation embryo development. a In vitro development of wild-type mouse zygotes microinjected with mRNA encoding WT or mutated CFP1. Time after superovulation was indicated. Scale bar, 100 μm. Arrows and hollow arrows indicated embryos arrested at 1- and 2-cell stages, respectively. b Quantification of developmental rates in (a). Error bars, S.E.M. c A diagram showing the function and regulation of CFP1 during oocyte meiotic maturation. In GV oocytes, SETD1–CFP1 complex mediates accumulation of H3K4me3, which is a permissive signal for haspin-mediated H3T3 phosphorylation upon meiotic resumption. During meiotic resumption, CDK1 triggers CFP1 phosphorylation and degradation. Phosphorylation weakened the interaction between CFP1 and histone H3, and ubiquitin–proteasome pathway-mediated degradation removes CFP1 from the condensing chromosomes. This dual inhibition mechanism maintains an appropriate H3K4me3 level that is essential for meiotic cell cycle progression

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