Fig. 4
Meiotic cell cycle progression in WT and Cxxc1oo−/− oocytes. a Live imaging results showing in vitro meiotic division of WT and Cxxc1oo−/− oocytes. Pre-MI pre-metaphase, MI metaphase I, MI-AI metaphase–anaphase transition, AI anaphase I, AI-MII anaphase I–metaphase II transition, MII metaphase II. Scale bar, 20 μm. b Data represent the mean and standard deviations of mCherry-securin fluorescence intensity levels in WT (blue) and Cxxc1oo−/− (red) oocytes at each time point. Time after GVBD is indicated. Values from individual oocytes were normalized relative to that at 5 h. Error bars, S.E.M. c SMC3 immunofluorescence (red) showing cohesin on chromosomes of WT and Cxxc1oo−/− oocytes at 16 h after in vitro culture. Centromeres were labeled by CREST staining (green). DNA was labeled by DAPI (blue). Scale bar, 5 μm
