Fig. 1

RNA-Seq reveals lincRNAs correlating with brown fat function in vivo. a, b Plot of BAT (a) mRNA or (b) lincRNA expression after HFD or CD feeding (RNA-Seq, n = 2 per biological condition). c, d Plot of BAT c mRNA or d lincRNA expression after 24 h of 4 °C or 22 °C (RNA-Seq, n = 2 per biological condition). e–g BAT, scWAT, and vWAT expression of e Elovl3 f Ucp1, and g H19 expression in CD-fed mice (n = 3–5 per given tissue, left), in CD-fed mice housed at 22 °C (n = 4) versus 24 h of 4 °C (n = 3, middle) and HFD-fed (n = 3 for Elovl3 and Ucp1, n = 7 for H19) versus CD-fed mice (n = 3 for Elovl3 and Ucp1, n = 7 for H19, right, qRT-PCR. h, i scWAT expression of Ucp1 and H19 in h mice housed at 22 °C (n = 2 for Ucp1, n = 4 for H19) versus 24 h of 4 °C (n = 2 for Ucp1, n = 4 for H19) and i HFD-fed (n = 4) versus CD-fed (n = 3 for Ucp1, n = 4 for H19) mice. j vWAT Ucp1 and H19 expression in mice housed at 22 °C versus 24 h of 4 °C (n = 2 for Ucp1, n = 4 for H19). Unless stated, bar graphs represent mean ± s.e.m. with all data points plotted and unpaired, two-tailed Student’s t-tests were used to assess statistical significance. *p < 0.05, **p < 0.01, ***p < 0.001. If applicable p-values are indicated within the panel