Fig. 5

Repression of BAT paternal monoallelic gene expression by the lincRNA H19. a Plot of expression fold-changes of maternally expressed genes (MEGs) in BAT versus a scWAT b vWAT. c Plot of expression fold-changes of paternally expressed genes (PEGs) between BAT versus c scWAT d vWAT. A Wilcoxon matched-pairs signed rank test was used to assess statistical significance for up- or downregulation of PEGs/MEGs (e) UCSC Genome Browser showing PEG abundances in BAT, scWAT, and vWAT. f, g Expression of indicated PEGs (f) or MEGS (g) in BAT 1° adipocytes transfected with scr or H19 LNA. f, g A paired, two-tailed Student’s t-test was used to assess significance across n = 4 experiments, n = 3 technical replicates each. h GSEA showing downregulation of PEGs but not MEGs (list from www.geneimprint.com) during 1°BAT differentiation. i, j Cumulative distribution frequency (left) and abundances (right) of PEGs in i 1°BAT and j 1°vWAT in siCtrl transfected adipocyte progenitors at d0 (black) versus differentiated siCtrl (blue) and siH19-transfected (red) 1° adipocytes at d3. k Quantification and overlap of H19 co-immunoprecipitating proteins in confluent (red) and differentiated (blue) PIBA cells determined by CHART-MS. Pulldown of H19-interacting proteins was performed using six H19-specific antisense/sense oligonucleotides in n = 3 replicates. l Illustration of H19 interaction network comprising n = 61 proteins generated using Ingenuity Pathway Analysis. Red and blue nodes depict proteins co-immunoprecipitating in confluent and differentiated cells, respectively. m AMIGO2 GO classification of H19 interactors across cellular states. n–p GSEA analysis of PEG gene ranks in mice n exposed to 4 °C cold stress for 24 h, o HFD feeding, or p exhibiting different susceptibility to DIO-evoked weight gains⁵³. q, r Correlation of WAT (q) H19 or (r) PEG abundances versus a ranked list of 24 obesity-prone and –resistant non-isogenic mouse strains. H19/PEG abundances were from (MOE430 V2)—Adipose (www.biogps.org54) and body composition after HFD feeding defined as obesity-resistant versus obesity-prone strains⁶⁵. Significance of association between expression versus (%) body fat was determined using Spearman’s correlation analysis. *p < 0.05, **p < 0.01, ***p < 0.001. If applicable additional p-values are indicated within the panel