Fig. 5

Mutation in rab-35 resulted in the similar phenotypes as lrk-1. a, b Venus BiFC fluorescence in wild-type and rab-35 transgenic models at day 13. The red arrowheads point to Venus BiFC-positive inclusions in pharynx. Twenty worms for each line were used (N = 3 in each group), * p < 0.05, Scale bars: 200 μm. c Worms with Venus BiFC-positive inclusions were quantified at the different ages. The color bars in graph (c) indicate percentages of Venus BiFC-positive inclusions. Thirty worms for each line were used (N = 3 in each group), * P < 0.05. d Quantification of worms that have axonal blebs. e Nerve fragmentation of each transgenic line at day 13. f Relative pharyngeal pumping rates at day 13. Thirty worms for each line were used (N = 3 in each group), * p < 0.05. All error bars represent SEM, P value, * p < 0.05, was measured by unpaired, two-tailed Student’s t test. g Mean life span. One hundred worms for each line were used (N = 3 in each group). F(3, 9)=33.730, P value,* p < 0.05, was calculated by one-way ANOVA with Tukey's post hoc test. All values are represented as mean ± SEM