Fig. 6

RAB35 Phosphorylation is necessary for regulation of α-synuclein propagation. a Venus BiFC fluorescence in Non-Transgenic (Non-Tg), hRAB35 WT, and hRAB35 T72A transgenic line at day 13. The red arrowheads indicate Venus BiFC-positive inclusions in pharynx. Scale bars: 200 μm. b Quantification of Venus BiFC fluorescence at day 13. Thirty worms for each line were used (N = 3 in each group). F(2, 6)=4.105, ns: not significant, * p < 0.05. c Worms with Venus BiFC-positive inclusions at day 13. The color bars in graph (c) show percentages of worms that have Venus BiFC-positive inclusions in rab-35 (tm2058) BiFC transgenic background. Thirty worms for each line were used (N = 3 in each group). F(2, 6)=4.670, ns: not significant, * p < 0.05. d, e Quantification of axonal bleb number (d) and fragmentation (e) at day 13. Thirty worms for each line were used (N = 3 in each group). F(2, 6)=5.607 (d), F(2, 6)=5.450 (e), ns: not significant, * p < 0.05. f Relative pharyngeal pumping rates at day 13. Thirty worms for each line were used (N = 3 in each group). F(2, 180)=4.421, ns: not significant, ** p < 0.01. g Mean life span (N = 3 in each group). One hundred worms for each line were used (N = 3 in each group). F(2, 6)=10.750, ns: not significant, ** p < 0.01. All values shown in figures are represented as mean ± SEM. P values, including ns: not significant, * p < 0.05, ** p < 0.01, were calculated by one-way ANOVA with Dunnet’s post hoc test