Fig. 5
Protein-protein interaction analysis of SseF and SscB by in vivo photocrosslinking a Protter39 visualization of SseF presenting predicted TM topology, chaperone binding domain (blue), and positions analyzed by in vivo photocrosslinking (orange). b Immunodetection of SseFFLAG in whole cell lysates with and without UV irradiation (upper panel). pBpa mutations are denotated as “X”. T3SS-dependent secretion of mutated SseF was analyzed by SDS PAGE, Western blotting and immunodetection of TCA-precipitated SseFFLAG in culture supernatants (lower panel). c As in b but showing SseFFLAG in a ΔsscB deletion background. d As in b but showing crosslinking position V73 of SseF in mutants harboring various leucine residues on the indicated positions of the first TMS of SseF. A representative result of three independent experiments is shown. CBD chaperone binding domain, WC whole cell lysates, sup culture supernatant, TMS transmembrane segment
