Fig. 5

Systemic neonatal but not adult injections of AAV8.EFS.mAsl exhibited long-term correction of the impaired NO metabolism in the brain. a Cortical argininosuccinate lyase (ASL) activity. b Levels of nitrite/nitrate levels in brain homogenates. c Computational quantification of nitrotyrosine immunostaining. d Representative images of nitrotyrosine-stained sections of cortex in WT, untreated, adult-injected and neonatally injected Asl^Neo/Neo mice (n = 3). Horizontal lines display the mean ± SEM. a−c One-way ANOVA with Dunnett’s post-test compared to WT: ns—not significant; *p < 0.05; **p < 0.01. b Graph displays not transformed data. Statistical analysis used log-transformed data. WT n = 6–8 (littermates aged 9 or 13 months); untreated Asl^Neo/Neo n = 5–6 (littermates aged 1–13 months as less than 10% of the animals had survived at the end of the study); adult-injected Asl^Neo/Neo n = 5 (aged 13 months); neonatally injected Asl^Neo/Neo