Fig. 6

Dual inhibition of PDGFR and VEGFR-2 inhibits glioma growth and progression. The primary GBM was induced in Ntv-a;Ink4a-Arf^−/−;Pten^−/−;LSL-Luc donor mice by RCAS-mediated somatic gene transfer. Single-cell tumor suspension was implanted into recipient mice, followed by treatment. a Schematic approach. b Animal survival was monitored for 70 days after injection (n = 6–7 mice). MS median survival. P values were determined by log-rank tests. c Tumor growth was analyzed by whole-body bioluminescence imaging. Left, representative images. Right, quantitative analysis of integrated luminescence in tumors at day 32–38 (n = 3–6, mean ± SEM). P values were determined by Student’s t test. d Tumor sections were stained with H&E and imaged. Representative data are shown from 3–4 mice/group. Scale bar: 100 μm. e Tumor sections were immunostained with anti-CD31 antibody and imaged. Shown are representative images (n = 3–5 mice). Scale bar: 100 μm