Fig. 5

Condition dependent read-through and TSS-dependent read-through. a Percentage of reading-through across TTS in Rich and M9 conditions. Colors denote the ratio between expression levels of transcripts in M9 vs Rich medium at TSS. b An example of the prs-dauA operon containing a previously known TTS (red arrow), where the rate of read-through is condition dependent. The TTS between the prs and dauA gene shows 91% read-through in Rich medium as opposed to 46% read-through in M9 medium. As a result the dauA gene is up-regulated in Rich medium compared to M9 growth condition despite comparable expression level between conditions for its co-transcribed prs gene. c An example of a TSS-dependent read-through for overlapping transcripts in the same orientations. 32% read-through is observed for the long transcript (TSS1) vs 5% read-through for the short transcript (TSS2). d Percentage of termination sites in M9 medium (red) and random positions (blue) with predicted attenuator structures (left panel) (Pasific algorithm²⁸) and predicted termination loop (right panel). 55 out of 408 termination sites have a predicted attenuator structure, and 386 of 408 termination sites have a predicted terminator structure