Fig. 1
From: A systems-level framework for drug discovery identifies Csf1R as an anti-epileptic drug target
Experimental plan and study overview. We studied 100 mice with epilepsy (pilocarpine post status epilepticus model of temporal lobe epilepsy) and 100 control (pilocarpine-naïve) matched littermate mice. At 4 weeks post status epilepticus, each mouse was continuously monitored using 3D accelerometry and video monitoring for 14 days to record seizure frequency and severity. High-throughput mRNA sequencing (RNA-seq) was generated using RNA from snap-frozen whole hippocampus samples from the mice and gene expression profiles were used to generate co-expression modules. Co-expression modules with a potential relationship to epilepsy were prioritized using the following criteria: (i) differential co-expression between epileptic and healthy hippocampus (mouse and human TLE), (ii) correlation of module expression with seizure frequency (mouse), and (iii) conservation in the human epileptic hippocampus. Modules meeting these criteria were considered candidate modules for epilepsy, and subjected to CRAFT analysis to identify membrane receptors predicted to restore disease module expression toward health
