Fig. 4 | Nature Communications

Fig. 4

From: CHD3 helicase domain mutations cause a neurodevelopmental syndrome with macrocephaly and impaired speech and language

Fig. 4

Restriction enzyme accessibility assay. a Restriction enzyme accessibility analysis of CHD3 wild-type and mutant proteins. 3.125, 6.25, or 12.5 nM of CHD3 proteins were incubated with 347 bp mono-nucleosomes. Digested fragments were analyzed by native polyacrylamide gel. b Quantitative analysis of restriction enzyme accessibility. Three individual experiments from two individual purifications (wild-type, p.Leu915Phe, p.Arg1121Pro, p.Asn1159Lys, p.Arg1172Gln, and p.Arg1187Pro) (N = 6) or one purification (p.Trp1158Arg) (N = 3) were conducted. The experimental data are presented as means with standard deviations

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