Fig. 1
Cell-cycle kinetics of green fluorescent protein (GFP)-labeled populations of DN3 thymocytes. a Experimental design for the analysis of cell-cycle kinetics of DN3 thymocytes. b Representative flow cytometric analysis of GFP expression in DN3 thymocytes from H2B-GFP;Lmo2Tg mice and littermate controls after labeling, followed by 0, 1, 2, 4 or 8 weeks of chase without doxycycline, as indicated. Unlabeled cells (neg), GFPlo and GFPhi populations are framed, with the average proportion (mean ± s.d.) of GFPhi cells indicated, Student’s t-test (vs control). c Kinetics of GFP-labeling loss in DN3 thymocytes from 6-week-old H2B-GFP (red) and H2B-GFP;Lmo2Tg mice (green), after the doxycycline pulse. d Progressive proportion of cell cycle-restricted cells within the GFPhi DN3 population from H2B-GFP;Lmo2Tg mice. Values are mean ± s.d., Student’s t-test, as compared to the 0 week post-pulse time point. e Cell-cycle analysis in GFPlo and GFPhi DN3 cells from H2B-GFP;Lmo2Tg mice, 2 weeks after removal of doxycycline. Values are mean ± s.e.m., Student’s t-test (vs control) *p < 0.05, **p < 0.01, ***p < 0.001
