Table 1 Primers used for genomic and quantitative PCR
Gene | Name of the primer | Sequence |
|---|---|---|
Cdkn1a (p21) | mCdkn1a_Fw | tacccgtctttccccagagt |
mCdkn1a_Rv | acactgtcacccacagatcg | |
Cdkn1b (p27) | mCdkn1b_F | gttagcggagcagtgtcca |
mCdkn1b_R | tctgttctgttggccctttt | |
Cdkn1c (p57) | mCdkn1c_F | caggacgagaatcaagagca |
mCdkn1c_R | gcttggcgaagaagtcgt | |
TcrB rearrangement | Name of the primer | Sequence |
mTcrb | S16 Fw | AGGAGCGATTTGCTGGTGTGG |
S16 Rv | GCTACCAGGGCCTTTGAGATG | |
Tcr Vb5 Fw | CCCAGCAGATTCTCAGTCCAACAG | |
Tcr Vb8 Fw | GCATGGGCTGAGGCTGATCCATTA | |
Tcr Jb2 Rv | TGAGAGCTGTCTCCTACTATCGATT | |
Genomic PCR of loci on Chr. 15 | Name of the primer | Sequence |
mIl7r | mIl7r Fw | ACTGTGGTTGGGTGCCTTAG |
mIl7r Rv | AAATTGGCAGAACCAACCAG | |
mMyc | mMyc Fw | ACACGGAGGAAAACGACAAG |
mMyc Fw | TCGTCTGCTTGAATGGACAG | |
Notch1 sequencing | Name of the primer | Sequence |
mNotch1 | Exon26_4853Fw | CCCTGTATGACCAGTA |
Exon26_5250Rv | ACGGATGTCCATGGGGT | |
Exon27_5253Fw | CTCCATTGTCTACCTG | |
Exon27_5401Rv | TCTTCACGGCCTCAATC | |
Exon34_6423Fw | TCCCCTGTTCCTGGCCG | |
Exon 34_6548Fw | TGCACCACGATATCGTG | |
Exon34_7265Fw | ATAGCATGATGGGGCCACTA | |
Exon 34_6849Fw | ACCCCATGGCTACTTGTCAG | |
Exon34_8157Rv | CTT CAC CCT GAC CAG GAA AA | |
Exon34_7284Rv | TAGTGGCCCCATCATGCTAT | |
Exon34_7675Rv | CCACAGGGGAGGAGGAGTAA | |
Exon34_8194Rv | TGA ATC CTT GTT CAT ATT TTA CAG ACA CAC AGG G |
