Fig. 4
From: microCLIP super learning framework uncovers functional transcriptome-wide miRNA interactions
Average PARS scores of AGO-bound regions deduced from the analysis of 4 EBV transformed lymphoblastoid PAR-CLIP libraries. RSS base signals were aligned to the start of the miRNA-target binding site. Base 0 corresponds to the 3′-end of the mRNA, at −1 or −2 nt downstream of the initiation of the direct miRNA seed pairing. Negative PARS scores correspond to single stranded RNA structures, while positive scores to double stranded sites. In the examined AGO-PAR-CLIP EF3D-AGO2 (a), LCL-BAC-D1 (b), LCL-BAC-D3 (c), and LCL-BAC (d) datasets, strong structural accessibility occurs in miRNA sites identified on T-to-C (red) and non-T-to-C (green) clusters in the 2–4 nt positions (yellow window) of the miRNA seed pairing. These results significantly differ from respective base scores along negative MREs (light blue) located on AGO-enriched peaks. Statistical significance of position structural changes was calculated using two-tailed Wilcoxon rank-sum test
