Fig. 1
From: Microbial metabolite sensor GPR43 controls severity of experimental GVHD
GPR43 protects against GVHD. B6 WT and Gpr43−/− mice received BMT from either syngeneic B6 or allogeneic BALB/c donors. a Gene expression of GPR43, GPR41, and GPR109a in IECs (CD326+) from syngeneic and allogeneic WT B6 recipients 7 and 14 days after BMT (n = 5 in Day 7, n = 6 in Day 14, two-tailed Mann–Whitney U test). b, c Representative immunoblots and densitometric analysis of GPR43 normalized to the presence of β-actin in IECs (CD326+) from syngeneic and allogeneic WT B6 recipients 14 days after BMT (n = 5 each, two-tailed Mann–Whitney U test). d, e Survival and clinical GVHD score after BMT treated with intragastric vehicle or GPR43 antagonists (10 mg kg−1 per day) from day 0 to day 21 (n = 3 each syngeneic, n = 6 allogeneic-vehicle, n = 5 allogeneic-antagonist, log-rank test for survival, two-tailed Mann–Whitney U test for GVHD Score). f–h Survival, weight change, and clinical GVHD score after BMT (n = 3 each syngeneic, n = 6 each, log-rank test for survival, two-tailed unpaired t-test for body weight change and two-tailed Mann–Whitney U test for GVHD Score). i, j Representative H&E stained colonic sections and histopathological GVHD score in colon and ileum from WT B6 or Gpr43−/− recipients on day 14 after BMT (scale bar, 100 μm) (n = 4 each syngeneic, n = 5 each allogeneic, two-tailed Mann–Whitney U test). k, l WT B6 and Gpr43−/− mice received BMT from either syngeneic B6 or allogeneic LP/J donors. Survival and clinical GVHD score after BMT (n = 5 each syngeneic, n = 10 each allogeneic, log-rank test for survival, two-tailed Mann–Whitney U test for GVHD Score). m, n WT B6 and Gpr43−/− mice received BMT from 129S1 donors. Survival and clinical GVHD score after BMT (129S1 BM to WT B6; n = 4, 129S1 BM to Gpr43−/−; n = 3, 129S1 BM + T cells to WT B6; n = 21, 129S1 BM + T cells to Gpr43−/−; n = 15, log-rank test for survival, two-tailed Mann–Whitney U test for GVHD Score). Data are representative of three experiments. *P < 0.05, **P < 0.01, ***P < 0.001, Bars and error bars show the mean ± s.e.m.
