Fig. 2
From: Sub-2 Å Ewald curvature corrected structure of an AAV2 capsid variant
Ewald sphere curvature correction improves the resolution of AAV2L336C and rotavirus VP6. ResLog60 plot of the a AAV2L336C dataset and b rotavirus VP6 dataset from Grant and Grigorieff37, with (green) and without (red) Ewald sphere curvature correction in Frealign9. Five replicates were performed for each data point with random particle distributions, and the standard deviation is plotted. Linear regression is plotted for the first linear portion of each set of points. The resolution limits predicted by Spence33,38, DeRosier30, and Downing & Glaeser32 for a 250 Å and 700 Å macromolecule respectively are indicated. c The theoretical resolution limits caused by curvature of the Ewald sphere at 300 keV plotted against particle diameter. Reconstructions from this study and a previous study35 that have shown an improvement in final resolution with Ewald sphere curvature correction are plotted (orange with and green without Ewald sphere curvature correction). The minimum resolutions at which noticeable improvements due to Ewald sphere curvature correction occur are also plotted in blue for AAV2L336C and rotavirus VP6, and follow closely with the Spence limit. d The theoretical resolution limits caused by the curvature of the Ewald sphere for particles smaller than 300 Å in diameter at 300 keV. Points on the Spence limit corresponding to 50, 100, 150, and 200 Å particle diameters are indicated and labeled in blue. Rest of the coloring scheme is the same as in c
