Table 1 Cryo-EM data collection and modeling statistics
From: Sub-2 Å Ewald curvature corrected structure of an AAV2 capsid variant
EM data collection/processing | AAV2L336C |
|---|---|
Microscope | FEI Titan Krios |
Voltage (kV) | 300 |
Camera | Gatan K2 Summit |
Nominal defocus range (μm) | 0.6–2 |
Defocus mean ± std (μm) | 1.1 ± 0.6 |
Exposure time (s) | 3.5 |
Dose rate (e− pixel−1 s−1) | 4 |
Total dose (e− Å−2) | 22.5 |
Pixel size (Å) | 0.788 |
Number of micrographs | 1317 |
Number of particles (processed) | 78,194 |
Number of particles (in final map) | 30,515 |
Symmetry | I |
Resolution limit during final refinement (Å) | 1.90 |
Resolution (global) (Å)a | 1.86 |
Local resolution range | 1.78–1.92 |
Directional resolution range | 1.86–1.86 |
Sphericity of 3DFSC | 1 |
Map sharpeningb | Spectral flattening between 8 and 1.8 Å, using pre-cut-off b-factor of −90 and post-cut-off b-factor of 0 |
Model statistics | AAV2L336C |
|---|---|
Residue range | 226–735 |
Map Cross Correlation | 0.849 |
Root Mean Square Deviation RMSD [bonds] (Å) | 0.01 |
Root Mean Square Deviation RMSD [angles] (Å) | 0.93 |
All-atom clashscore | 7.97 |
Ramachandran plot | |
Favored (%) | 97.2 |
Allowed (%) | 2.8 |
Outliers | 0 |
Rotamer outliers | 0 |
C-β deviations | 0 |
EM-Ringer score | 8.23 |
Average b-factor | 16.1 |
