Fig. 10

CD2/CD58 and LFA-1/ICAM-1 play important roles in TMP-mediated DC activation. a DCs (5 × 10⁶) were treated with either PBS or 4-TMPs (5 × 10⁷) for 2 h, and the transfer of indicated proteins on DCs (CD11c⁺) was analysed by flow cytometry. Data represent the mean of three experiments ± SEM. *P < 0.01 vs. no 4-TMPs. b Fluo-3-loaded DCs (5 × 10⁶) were pretreated for 30 min with the indicated blocking antibodies (anti-CD2, -CD40, and -LFA-1), and then stimulated with 4-TMPs (5 × 10⁷). Calcium signals were expressed as arbitrary units (a.u.) of Fluo-3 fluorescence from the average of at least three separate experiments. Data represent the mean of three experiments ± SEM. *P < 0.01 vs. PBS-treated group. c Representative images of cytokine arrays (left) and the heatmap showing cytokine-expression levels in total CD4⁺ T cell lysate and 4-TMPs (right). Each sample (200 µg) was hybridized to membranes containing capture antibodies specific for 112 cytokines. Data were quantified by densitometric analysis and statistically analysed. Cytokines overexpressed by up to 1.5-fold in TMPs relative to their expression in lysate are highlighted by red and blue boxes. Some cytokines were further confirmed by ELISA. Data represent the mean of three experiments ± SEM. *P < 0.01 vs. CD4⁺ T cell lysate