Fig. 3
From: Crystal structure of TcpK in complex with oriT DNA of the antibiotic resistance plasmid pCW3
The crystal structure of TcpK in complex with oriTmin23. a Ribbon representation of the overall structure of the complex. For clarity, only one half of the asymmetric unit is represented. Two orthogonal views along the vertical axis are presented. Each TcpK monomeric subunit is colored differently and DNA molecules are represented as surface structures. b Schematic representation of the contacts between TcpK and the DNA molecule. The respective chain IDs are presented in parentheses. For clarity, only hydrogen bond interactions are depicted and colored in blue, letters corresponding to the TcpK box nucleotides are colored in red. c Closer view of the DNA binding region. Selected residues of TcpK making hydrogen bond interactions with the DNA are represented as sticks. d Continuous sedimentation coefficient (c(s)) distribution for 2 μM oriTmin23 and e for 24 μM TcpK in the presence of 12 μM oriTmin23 calculated from the best fit to sedimentation data shown in Supplementary Figure 3d, e. f Continuous sedimentation coefficient (c(s)) distribution for 24 μM TcpKRep in the presence of 12 μM oriTmin23 calculated from the best fit to the sedimentation data shown in Supplementary Figure 3g
