Fig. 4
From: Crystal structure of TcpK in complex with oriT DNA of the antibiotic resistance plasmid pCW3
Binding analysis of TcpK binding to inter TcpK box linker sequence nucleotide derivatives. The 23 bp DNA sequence identified from the SPR mapping experiment has -CAG- as TcpK box linker sequence. Nucleotide substitutions at the 3-nt linker region are indicated on the graph and -Δ- is a deletion of all three bases. TcpK binding to each fragment was tested with protein concentrations of 1 μM (checked bars) and 0.1 μM (solid bars). The responses are expressed as a percentage of theoretical Rmax
