Fig. 6
Somatically matured CA45 bridges the termini of the cathepsin cleavage loop. a A close-up view of CA45 bound to EBOV GPΔMuc, with Cα atoms of residues that flank germline deletion G31GLΔ shown in red spheres. CA45 mediates interactions with both termini (green and yellow spheres) of the disordered cathepsin loop (dashed line). b Sequence alignment of mature CA45 heavy and light chains against cynomolgus macaque germline precursors. Residues that undergo somatic mutation in CA45 are shaded in cyan and those that are buried at the interface with GP1 or GP2 are labeled with green and orange asterisks, respectively. Asterisks shaded yellow mediate interface with glycan cap. c CA45 paratope with full GP interface mapped onto CA45 Fab shown in surface representation (heavy chain, purple; light chain, gray), rotated by 90° from view in a. Footprints of GP1 are shown in green, of glycan cap yellow, and of GP2 orange. d CA45 residues that are somatically hypermutated (SHM) are mapped onto CA45 surface in cyan with unmutated heavy and light chain residues colored in purple and gray, respectively. Shown in same orientation as in c. Residues that flank the G31GLΔ germline deletion are shown in red
