Fig. 1

Loss of NLRP1 confers protection against DSS-induced colitis. WT and Nlrp1^−/− mice were given 3% (w/v) DSS for 6 days followed by normal drinking water for 2 days and disease severity was measured according to a percentage weight loss, b colon length and c histology score for epithelial damage and inflammatory cell infiltrate of hematoxylin and eosin (H&E)-stained sections of the colon, scale bar = 1 mm. d Representative H&E-stained sections of the colon from WT and Nlrp1^−/− mice. Lethally irradiated WT and Nlrp1^−/− mice were reconstituted with WT or Nlrp1^−/− bone marrow for 12 weeks and were given 3% DSS for 6 days. Disease severity was measured by e percentage weight loss, f colon length and g histology score. Data are representative of 3 independent experiments with 3–5 mice per group. Means ± SEM; *p ≤ 0.05, **p ≤ 0.01, ***p ≤ 0.001, ****p ≤ 0.0001. As determined by a two-tailed, unpaired t-test. A one-way analysis of variance (ANOVA) and Tukey’s post-hoc comparisons were performed on data that involved more than two comparisons