Fig. 1
From: Somatic Trp53 mutations differentially drive breast cancer and evolution of metastases
Generation and characterization of the Trp53wm-R172H and Trp53wm-R245W alleles. a Schematic representation of the Trp53wm-R172H and Trp53wm-R245W alleles. The cre-loxP-mediated strategy was used to generate the Trp53R172H and Trp53R245W alleles. Red triangle loxP site; blue rectangle Frt site left after removing the selection markers (see Supplementary Fig. 1); A, Trp53 native polyadenylation signaling sequence; * 515G->C mutation was introduced in exon5 for the Trp53wm-R172H allele, and 733 C→T and 735 C→G mutations were introduced in exon 7 for the Trp53wm-R245W allele. b Western blot analysis for p53 protein levels in thymuses of various Trp53 genotypes (WT, wm-R172H/+ and wm-R245W/+). IR, γ-radiation. c Real-time reverse transcription PCR analysis for Cdkn1a and Bbc3 mRNAs in thymuses of various Trp53 genotypes (WT, wm-R172H/+ and wm-R245W/+). Light blue circles, mRNA level of Cdkn1a or Bbc3 in non-irradiated thymuses; Dark blue squares, mRNA level of Cdkn1a or Bbc3 in irradiated thymuses. Error bars, s.d. d Quantification of the normalized number of γ-radiation-induced apoptotic thymocytes of various Trp53 genotypes (WT, wm-R172H/+ and wm-R245W/+), following annexin V staining and FACS analysis. Error bars, s.d. e Quantification of cells in S phase for γ-irradiated and non-irradiated MEFs of various Trp53 genotypes (WT, wm-R172H/+ and wm-R245W/+). Error bars, s.d. f Kaplan–Meier survival curves of Trp53 WT (n = 23), Trp53wm-R172H/+ (n = 42) and Trp53wm-R245W/+ (n = 15) mice. g Kaplan–Meier survival curves of Trp53+/− n = 23) and Trp53wm-R172H/- (n = 33) mice. In c–g, no statistical differences were observed between samples with different Trp53 genotypes (WT, wm-R172H/+ and wm-R245W/+)
