Fig. 3
From: Somatic Trp53 mutations differentially drive breast cancer and evolution of metastases
Characteristics of the p53R172H and p53R245W mammary tumors a–e Hematox;ylin and Eosin-stained sections of mammary tumors from mice showing different pathological subtypes. a A moderately-differentiated adenocarcinoma. Note glandular and nesting formation of tumor composition; b A highly anaplastic cellular manifestation including spindle cell, cellular pleomorphism and loss of glandular and differentiation features; c A sarcomatoid carcinoma with an undifferentiated sarcoma-like manifestation. d A localized metastatic adenocarcinoma in lung. Tumor nodule displays glandular proliferation of well-to-moderately-differentiated adenocarcinoma of mammary origin; e A metastatic tumor to liver. The tumor displays the same phenotypic anaplasia seen in primary tumor (b). Note the distinct boundary of metastases and normal tissues, as marked by the dashed line. Scale bar is 50 μm and applies to all sections. f RT-qPCR analysis for Esr1, Pgr and Erbb2 in WT mouse spleens, mammary glands (MG), or ovaries, or mammary tumors from the MMTV-Erbb2 mice, and tumors from Ad-Cre-injected mice of various Trp53 genotypes (wm172/+, wm-R172H/+; wm172/fl, wm-R172H/fl; wm245/+, wm-R245W/+). IR, γ-radiation. Error bars, s.d. g Summary of the molecular subtypes of mammary tumors from mice of various genotypes, based on data in f. Esr1/Pgr+ label means Esr1 postive or Pgr positive, or both. h RT-qPCR analysis for Fscn1, Ltbp1, and Cxcr4, in Trp53R245W-driven mammary tumors compared to Trp53R172H mammary tumors. Error bars, s.d. * p < 0.05 (t-test) i RT-qPCR analysis for Sparc in Trp53R245W-driven mammary tumors that had metastasis compared to those without metastasis. Error bars, s.d. * p < 0.05 (t-test)
