Fig. 2
From: Lineage dynamics of murine pancreatic development at single-cell resolution
Identification of multiple uncharacterized mesenchymal populations. a t-SNE visualization of subclustered E14.5 mesenchymal clusters (from n = 14 pancreata). b Density plot depicting Pearson’s correlation values (depicted in heatmap in Supplementary Fig. 3b) within the epithelial and mesenchymal populations based on average gene expression in each cluster. c Dot plot of top differentially expressed markers of each mesenchymal population. Bars are color-coded by cluster identity in a. The gray bar represents pan-mesenchymal markers. The size of each dot represents the proportion of cells within a given population that expresses the gene; the intensity of color indicates the average level of expression. d Pathway analysis of genes greater than 2-fold differentially expressed by cells in clusters 1, 2, 4, and 5. e Expression of genes marking clusters 1 (Cav1), 2 (Stmn2), 4 (Cxcl12), and 5 (Barx1) in all E14.5 mesenchymal cells. Color intensity indicates level of expression. f–h Multiplexed fluorescent ISH combined with Epcam IF validates clusters 2 and 5 (f) and cluster 1 (g-h). Epcam marks pancreatic epithelium. In (f), Barx1 + cells (red arrows, cluster 5) are distinct from Stmn2+ cells (green arrows, cluster 2), validating the single-cell data. In (g), Cav1+ cells (red arrows, cluster 1) are distinct from Stmn2+ cells (green arrows, cluster 2). In (h), Barx1+ cells that do not express Cav1 (red arrows) represent cluster 5, whereas Barx1+/Cav1+ cells (yellow arrows) represent cluster 1. Cav1+ cells that do not express Barx1 are also identified (green arrows), likely representing endothelial cells79. Scale bar represents 50 µm in f–h
